by AAB Research

Primer design tools

Free browser versions of the AAScan and PCRCloning programs, after a quick registration. Everything runs locally in your browser, and your sequences are never uploaded.

AAScan

Alanine-scanning mutagenesis. Designs partially overlapping primer pairs for every residue in a range (Ala→Gly, everything else→Ala).

PCR Cloning

Inserts a PCR fragment into a vector with overlapping ends. Designs vector and insert primers with overhangs on the insert, on the vector, balanced between them, or as megaprimers.

Gibson assembly and cloning seminars from AAB Research

We also offer Gibson assembly products and cloning seminars. To hear about them, tick the box when you register, or later under Preferences.