:root {
  --bg: #f5f6f8;
  --panel: #ffffff;
  --ink: #1b2330;
  --muted: #647083;
  --line: #dfe3ea;
  --accent: #1f6f78;
  --accent-ink: #ffffff;
  --accent-soft: #e2f1f2;
  --fwd: #1f6f78;
  --rev: #9a4d1f;
  --bad: #b3261e;
  --bad-soft: #fbe9e8;
  --sel: #fff1c2;
  --warn: #8a5a00;
  --warn-soft: #fff5dc;
  --seg-vector: #5b6b82;
  --seg-extra: #8b4fb3;
  --seg-insert: #1f6f78;
  --mono: ui-monospace, "SF Mono", Menlo, Consolas, "Liberation Mono", monospace;
  --sans: system-ui, -apple-system, "Segoe UI", Roboto, Helvetica, Arial, sans-serif;
  color-scheme: light;
}
@media (prefers-color-scheme: dark) {
  :root:not([data-theme="light"]) {
    --bg: #12161c;
    --panel: #1a2029;
    --ink: #e6eaf0;
    --muted: #93a0b3;
    --line: #2c3542;
    --accent: #4fb3bd;
    --accent-ink: #0d1a1c;
    --accent-soft: #1d3336;
    --fwd: #5cc0c9;
    --rev: #e59a66;
    --bad: #ff8a80;
    --bad-soft: #3a1f1e;
    --sel: #4a3f14;
    --warn: #f0c060;
    --warn-soft: #3a3016;
    --seg-vector: #8d9bb0;
    --seg-extra: #c08ae3;
    --seg-insert: #4fb3bd;
    color-scheme: dark;
  }
}

* { box-sizing: border-box; }
html, body { margin: 0; }
body {
  background: var(--bg);
  color: var(--ink);
  font: 14px/1.45 var(--sans);
}

/* ---------- top bar ---------- */
.topbar {
  display: flex; align-items: center; justify-content: space-between; gap: 12px; flex-wrap: wrap;
  padding: 10px 20px; background: var(--panel); border-bottom: 1px solid var(--line);
}
.brand { display: flex; align-items: baseline; gap: 12px; flex-wrap: wrap; }
.logo { font-size: 20px; letter-spacing: .02em; color: var(--accent); text-decoration: none; }
.toolnav { display: flex; gap: 2px; background: var(--bg); border: 1px solid var(--line); border-radius: 8px; padding: 2px; }
.toolnav a { color: var(--muted); text-decoration: none; padding: 3px 10px; border-radius: 6px; font-size: 13px; }
.toolnav a:hover { color: var(--ink); }
.toolnav a[aria-current="page"] { background: var(--panel); color: var(--ink); font-weight: 600; box-shadow: 0 0 0 1px var(--line); }
.logo b { color: var(--ink); }
.subtitle { color: var(--muted); }
.actions { display: flex; gap: 6px; flex-wrap: wrap; }

/* ---------- layout ---------- */
.layout {
  display: grid; gap: 16px; padding: 16px 20px;
  grid-template-columns: minmax(0, 1fr) 360px;
  grid-template-areas: "seq params" "results params";
  align-items: start;
}
.seq-panel { grid-area: seq; }
.params-panel { grid-area: params; position: sticky; top: 16px; }
.results-panel { grid-area: results; }
@media (max-width: 980px) {
  .layout { grid-template-columns: minmax(0, 1fr); grid-template-areas: "seq" "params" "results"; padding: 12px 16px; }
  .params-panel { position: static; }
}

.panel { background: var(--panel); border: 1px solid var(--line); border-radius: 10px; padding: 14px 16px; min-width: 0; }
.panel-head { display: flex; align-items: baseline; gap: 12px; flex-wrap: wrap; margin-bottom: 10px; }
h2 { font-size: 15px; margin: 0; }
h3 { font-size: 13px; margin: 0; }
.hint, .muted { color: var(--muted); font-size: 12.5px; }
p.hint { margin: 6px 0 0; }

/* ---------- inputs ---------- */
textarea, input[type="text"], input[type="number"] {
  font: inherit; color: var(--ink); background: var(--bg);
  border: 1px solid var(--line); border-radius: 6px; padding: 5px 7px; width: 100%;
}
textarea { font-family: var(--mono); font-size: 12.5px; resize: vertical; line-height: 1.5; }
#seq { word-break: break-all; }
input:focus, textarea:focus { outline: 2px solid var(--accent); outline-offset: -1px; }
input.codon { font-family: var(--mono); text-transform: uppercase; letter-spacing: .08em; }
input.small { width: 70px; }

fieldset { border: 0; border-top: 1px solid var(--line); margin: 0; padding: 10px 0 12px; }
fieldset:first-of-type { border-top: 0; padding-top: 0; }
legend { font-weight: 600; font-size: 12px; text-transform: uppercase; letter-spacing: .06em; color: var(--muted); padding: 0 0 6px; }
.grid { display: grid; gap: 8px 10px; }
.g2 { grid-template-columns: repeat(2, minmax(0, 1fr)); }
.g3 { grid-template-columns: repeat(3, minmax(0, 1fr)); }
.grid label { display: flex; flex-direction: column; gap: 3px; font-size: 12.5px; color: var(--muted); }
label.check { display: flex; align-items: center; gap: 6px; margin-top: 8px; }
label.inline { display: inline-flex; align-items: center; gap: 6px; margin-top: 8px; color: var(--muted); font-size: 12.5px; }
label.inline input { width: 80px; }
.adaptive { display: flex; align-items: center; gap: 6px; }
.adaptive label.check { margin-top: 8px; }
.adaptive input { margin-top: 8px; }

.seg { display: inline-flex; border: 1px solid var(--line); border-radius: 7px; overflow: hidden; flex-wrap: wrap; }
.seg label { cursor: pointer; }
.seg input { position: absolute; opacity: 0; pointer-events: none; }
.seg span { display: block; padding: 5px 11px; font-size: 12.5px; border-right: 1px solid var(--line); }
.seg label:last-child span { border-right: 0; }
.seg input:checked + span { background: var(--accent); color: var(--accent-ink); }
.seg input:focus-visible + span { outline: 2px solid var(--accent); outline-offset: -2px; }

/* ---------- buttons ---------- */
button, .button {
  font: inherit; font-size: 13px; cursor: pointer; border-radius: 6px;
  padding: 6px 12px; border: 1px solid var(--line); background: var(--panel); color: var(--ink);
  display: inline-flex; align-items: center;
}
button:hover:not(:disabled), .button:hover { border-color: var(--accent); }
button:disabled { opacity: .45; cursor: default; }
button.primary {
  width: 100%; justify-content: center; margin-top: 6px; padding: 9px 12px;
  background: var(--accent); color: var(--accent-ink); border-color: var(--accent); font-weight: 600;
}
button.primary:hover { filter: brightness(1.08); }

.errors { color: var(--bad); font-size: 13px; }
.errors:not(:empty) { margin-top: 10px; background: var(--bad-soft); border-radius: 6px; padding: 8px 10px; }
.errors ul { margin: 0; padding-left: 18px; }

/* ---------- sequence meta / frame ---------- */
.seq-meta { display: flex; justify-content: space-between; align-items: center; gap: 10px; flex-wrap: wrap; color: var(--muted); font-size: 12.5px; }
.frame { margin-top: 8px; }
.frame summary { cursor: pointer; font-size: 13px; }
.protein { font-family: var(--mono); font-size: 12px; margin-top: 6px; max-height: 190px; overflow: auto; }
.prow { display: flex; gap: 10px; white-space: nowrap; }
.pnum { color: var(--muted); min-width: 3.5em; text-align: right; }
.paa span.gap { margin-right: .6em; }
.paa span.sel { background: var(--sel); }
.paa span.stop { color: var(--bad); font-weight: 700; }

/* ---------- results ---------- */
.stats { display: flex; gap: 16px; margin-left: auto; flex-wrap: wrap; }
.stat { display: flex; align-items: baseline; gap: 5px; font-size: 12.5px; color: var(--muted); }
.stat b { font-size: 15px; color: var(--ink); font-variant-numeric: tabular-nums; }
.stat.bad b { color: var(--bad); }

.tabs { display: flex; gap: 2px; border-bottom: 1px solid var(--line); margin-bottom: 10px; }
.tabs button { border: 0; border-bottom: 2px solid transparent; border-radius: 0; background: none; padding: 6px 12px; color: var(--muted); }
.tabs button.active { color: var(--ink); border-bottom-color: var(--accent); font-weight: 600; }

.table-tools { display: flex; justify-content: flex-end; margin-bottom: 8px; }
.table-wrap { overflow: auto; max-height: 70vh; border: 1px solid var(--line); border-radius: 8px; }
table { border-collapse: collapse; width: 100%; font-size: 12.5px; }
thead th {
  position: sticky; top: 0; background: var(--panel); z-index: 1;
  text-align: left; font-weight: 600; color: var(--muted); padding: 7px 8px; border-bottom: 1px solid var(--line); white-space: nowrap;
}
td { padding: 4px 8px; vertical-align: middle; white-space: nowrap; }
tr.group-start td { border-top: 1px solid var(--line); }
tbody tr:first-child td { border-top: 0; }
td.mut { font-weight: 600; font-family: var(--mono); vertical-align: top; padding-top: 6px; }
td.mut.alt { color: var(--muted); font-weight: 400; }
td.mut .tag { display: block; font-weight: 400; font-size: 10.5px; color: var(--rev); }
td.strand { font-weight: 700; font-family: var(--mono); }
td.strand.F { color: var(--fwd); }
td.strand.R { color: var(--rev); }
td.num { text-align: right; font-variant-numeric: tabular-nums; }
td.seqcell { font-family: var(--mono); font-size: 12px; }
.oligo b { background: var(--accent-soft); color: var(--accent); border-radius: 2px; }
tr.fail td.failmsg { color: var(--bad); }
tr.empty td { text-align: center; color: var(--muted); padding: 30px; white-space: normal; }

.out-block + .out-block { margin-top: 12px; }
.out-head { display: flex; align-items: center; gap: 6px; margin-bottom: 6px; }
.spacer { flex: 1; }
.out-block textarea { white-space: pre; overflow-wrap: normal; overflow-x: auto; }

.foot { color: var(--muted); font-size: 12px; padding: 4px 20px 24px; }
.foot a { color: var(--accent); }

textarea.wrap { white-space: pre-wrap; word-break: break-all; }

/* ---------- PCR cloning ---------- */
.segments { display: flex; flex-direction: column; gap: 10px; }
.segin { display: flex; flex-direction: column; gap: 4px; }
.seghead { display: flex; align-items: baseline; gap: 6px; font-size: 13px; font-weight: 600; flex-wrap: wrap; }
.seghead .hint { font-weight: 400; }
.seghead i { width: 10px; height: 10px; border-radius: 2px; align-self: center; flex: none; }
.seghead .count { margin-left: auto; color: var(--muted); font-weight: 400; font-size: 12px; font-variant-numeric: tabular-nums; }
.segin textarea { word-break: break-all; border-left-width: 3px; }
.segin.vector5 i, .segin.vector3 i { background: var(--seg-vector); }
.segin.vector5 textarea, .segin.vector3 textarea { border-left-color: var(--seg-vector); }
.segin.extra5 i, .segin.extra3 i { background: var(--seg-extra); }
.segin.extra5 textarea, .segin.extra3 textarea { border-left-color: var(--seg-extra); }
.segin.insert i { background: var(--seg-insert); }
.segin.insert textarea { border-left-color: var(--seg-insert); }

.warnings { color: var(--warn); font-size: 13px; }
.warnings:not(:empty) { background: var(--warn-soft); border-radius: 6px; padding: 8px 10px; margin-bottom: 10px; }
.warnings ul { margin: 0; padding-left: 18px; }

.set-bar { display: flex; align-items: center; gap: 6px; flex-wrap: wrap; }
.set-desc { margin: 8px 0 10px; }

.map { position: relative; margin: 6px 0 14px; padding: 0 2px; }
.map-track { position: relative; height: 22px; border-radius: 4px; background: var(--bg); }
.map-seg { position: absolute; top: 0; bottom: 0; color: #fff; font-size: 11px; line-height: 22px; text-align: center; overflow: hidden; white-space: nowrap; }
.map-seg.vector5, .map-seg.vector3 { background: var(--seg-vector); }
.map-seg.extra5, .map-seg.extra3 { background: var(--seg-extra); }
.map-seg.insert { background: var(--seg-insert); }
.map-seg:first-child { border-radius: 4px 0 0 4px; }
.map-seg:last-child { border-radius: 0 4px 4px 0; }
.map-lane { position: relative; height: 26px; }
.map-primer { position: absolute; height: 6px; border-radius: 3px; }
.map-lane.fwd .map-primer { bottom: 4px; }
.map-lane.rev .map-primer { top: 4px; }
.map-primer.F { background: var(--fwd); }
.map-primer.R { background: var(--rev); }
.map-primer span { position: absolute; left: 50%; transform: translateX(-50%); font-size: 11px; font-family: var(--mono); white-space: nowrap; }
.map-lane.fwd .map-primer span { bottom: 7px; color: var(--fwd); }
.map-lane.rev .map-primer span { top: 7px; color: var(--rev); }

.junctions { display: flex; flex-direction: column; gap: 12px; margin-top: 14px; }
.junction h3 { margin-bottom: 6px; display: flex; gap: 10px; align-items: baseline; flex-wrap: wrap; }
.ovl-tag { font-weight: 600; font-size: 12px; color: var(--ink); background: var(--sel); border-radius: 4px; padding: 1px 7px; }
.aln-seg.ovl { background: var(--sel); color: var(--ink); }
.subhead { margin: 16px 0 6px; }
td.low { color: var(--bad); font-weight: 600; }
td.note { color: var(--muted); font-size: 12px; }
.nn-only { margin-top: 10px; }
.cond-head { font-size: 12px; font-weight: 600; margin: 10px 0 6px; display: flex; align-items: center; gap: 8px; }
.linkish { border: 0; background: none; padding: 0; color: var(--accent); font-size: 12px; text-decoration: underline; cursor: pointer; }
.nn-only[hidden] { display: none; }
input:disabled { opacity: .5; }
.aln { font-family: var(--mono); font-size: 12px; line-height: 1.55; overflow-x: auto; border: 1px solid var(--line); border-radius: 8px; padding: 8px 10px; background: var(--bg); }
.aln-row { display: flex; white-space: pre; }
.aln-label { flex: none; width: 7.5em; color: var(--muted); }
.aln-row, .aln-row * { font: inherit; }   /* one font size/weight for every line so columns stay in register */
.aln-bottom { color: var(--muted); }
.aln-primer.F { color: var(--fwd); font-weight: 600; }
.aln-primer.R { color: var(--rev); font-weight: 600; }
.aln-seg.vector5, .aln-seg.vector3 { color: var(--seg-vector); }
.aln-seg.extra5, .aln-seg.extra3 { color: var(--seg-extra); }
.aln-seg.insert { color: var(--seg-insert); }

/* ---------- landing ---------- */
.landing { max-width: 880px; margin: 0 auto; padding: 32px 20px; }
.landing h1 { font-size: 22px; margin: 0 0 6px; }
.cards { display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 16px; margin-top: 20px; }
.card { display: block; text-decoration: none; color: inherit; background: var(--panel); border: 1px solid var(--line); border-radius: 10px; padding: 18px; }
.card:hover { border-color: var(--accent); }
.card h2 { font-size: 16px; margin-bottom: 6px; color: var(--accent); }
.card p { margin: 0; color: var(--muted); }

/* ---------- registration / account ---------- */
.account { display: flex; align-items: center; gap: 6px; flex-wrap: wrap; min-width: 0; max-width: 100%; }
.account:empty { display: none; }
.account .who { color: var(--muted); font-size: 12.5px; max-width: min(220px, 100%); overflow: hidden; text-overflow: ellipsis; white-space: nowrap; }
a.button { text-decoration: none; }

.auth-wrap { max-width: 980px; margin: 0 auto; padding: 28px 20px; display: grid; gap: 24px; grid-template-columns: minmax(0, 1fr) minmax(0, 420px); align-items: start; }
@media (max-width: 820px) { .auth-wrap { grid-template-columns: minmax(0, 1fr); padding: 16px; } }
.auth-intro h1 { font-size: 24px; margin: 0 0 10px; }
.auth-intro p { color: var(--muted); }
.promo { background: var(--accent-soft); border-radius: 10px; padding: 14px 16px; margin-top: 16px; }
.promo h2 { font-size: 15px; margin: 0 0 6px; }
.promo ul { margin: 6px 0; padding-left: 20px; }
.promo.wide { margin-top: 24px; }
.promo.wide p { margin: 0; }
.auth-card form { display: flex; flex-direction: column; gap: 10px; }
.auth-card .grid label, label.stack { display: flex; flex-direction: column; gap: 3px; font-size: 12.5px; color: var(--muted); }
.auth-card input[type="email"], .auth-card input[type="text"] { font-size: 14px; padding: 7px 9px; }
label.check.consent { align-items: flex-start; font-size: 13px; line-height: 1.4; margin-top: 2px; }
label.check.consent input { margin-top: 3px; flex: none; }
.interests { padding: 4px 0 0 24px; border: 0; }
.interests legend { text-transform: none; letter-spacing: 0; font-size: 12.5px; padding: 0; }
.interests label.check { margin-top: 4px; }
.interests:disabled { opacity: .5; }
input.invalid { border-color: var(--bad); outline: 1px solid var(--bad); }
.hp { position: absolute; left: -10000px; width: 1px; height: 1px; overflow: hidden; }

dialog.prefs { border: 1px solid var(--line); border-radius: 12px; background: var(--panel); color: var(--ink); max-width: 440px; width: calc(100% - 32px); padding: 18px 20px; }
dialog.prefs::backdrop { background: rgb(0 0 0 / .35); }
dialog.prefs h2 { margin-bottom: 4px; }
.dlg-actions { display: flex; justify-content: flex-end; gap: 8px; margin-top: 12px; }
.dlg-actions button.primary { width: auto; margin-top: 0; }

.doc { max-width: 760px; margin: 0 auto; padding: 28px 20px; }
.doc h1 { font-size: 24px; margin: 0 0 4px; }
.doc h2 { font-size: 16px; margin: 22px 0 6px; }
.doc li { margin: 4px 0; }
.todo { background: var(--warn-soft); color: var(--warn); border-radius: 3px; padding: 0 3px; font-weight: 600; }

.admin { padding: 16px 20px; }
.admin-tools { display: flex; gap: 10px; align-items: center; flex-wrap: wrap; margin-bottom: 6px; }
.admin-tools input[type="search"] { max-width: 320px; font: inherit; color: var(--ink); background: var(--bg); border: 1px solid var(--line); border-radius: 6px; padding: 5px 8px; }
td.center { text-align: center; }
button.danger { color: var(--bad); }
button.danger:hover { border-color: var(--bad); }

/* admin: interest stats */
.barwrap { width: 34%; min-width: 90px; }
.barcell { background: var(--bg); border-radius: 3px; height: 9px; overflow: hidden; }
.barcell span { display: block; height: 100%; background: var(--accent); border-radius: 3px; }
td.hot { color: var(--accent); font-weight: 700; }
.admin-tools select { font: inherit; color: var(--ink); background: var(--bg); border: 1px solid var(--line); border-radius: 6px; padding: 5px 8px; }